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J Med Virol 41: 55-60 (1993)[94045587]
Detection of hepatitis C virus RNA in peripheral blood
mononuclear cells and in saliva
K. C. Young, T. T. Chang, T. C. Liou & H. L. Wu
Department of Medical Technology, National Cheng Kung University,
Tainan, Taiwan, Republic of China.
The nested polymerase chain reaction (PCR) technique was applied
to investigate hepatitis C virus (HCV) RNA in the peripheral blood
mononuclear cells (PBMCs), saliva, and serum of patients with
chronic type C hepatitis. The specificity of the amplified products
was analyzed and confirmed by agarose gel electrophoresis, Southern
blot hybridization, and restriction endonuclease pattern analysis.
HCV RNA was detectable in the PBMCs of 24% (12/50) of the patients.
The HCV RNA detected in PBMCs was not due to the contamination from
plasma, since no viral sequences could be detected in the third
washing of PBMCs. Of the 12 patients with HCV RNA in PBMCs, five
were negative for HCV RNA sequences in the serum. Thus the presence
of HCV RNA in PBMCs was not strictly correlated to the results for
sera. Among 25 patients with HCV RNA in their saliva, 18 were
negative for PBMCs. Among 25 patients without HCV RNA in their
saliva, five had HCV RNA in PBMCs. In conclusion, PBMCs are an
extrahepatic target for chronic HCV infection. However, we do not
suggest that PBMCs act as a vehicle for carrying HCV to saliva,
since the presence of HCV RNA in PBMCs and in saliva was not
closely correlated.
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