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NEW HEPATITIS C VIRUS (HCV) GENOTYPING SYSTEM THAT ALLOWS FOR
IDENTIFICATION OF HCV GENOTYPE-1A, GENOTYPE-1B, GENOTYPE-2A,
GENOTYPE-2B, GENOTYPE-3A, GENOTYPE-3B, GENOTYPE-4, GENOTYPE-5A, AND
GENOTYPE-6A
Recent studies have focused on whether different hepatitis C
virus (HCV) genotypes are associated,vith different profiles of
pathogenicity, infectivity, and response to antiviral therapy. The
establishment of a simple and precise genotyping system for HCV is
essential to address these issues, A new genotyping system based on
PCR of the core region with genotype-specific PCR primers for the
determination of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and
6a was developed, A total of 607 samples (379 from Japan, 63 from
the United States, 53 from Korea, 35 from Taiwan, 32 from China, 20
from Hong Kong, 15 from Australia, 6 from Egypt, 3 from Bangladesh,
and 1 from South Africa) were tested by both the assay of Okamoto
et al. (H. Okamoto, Y. Sugiyama, S. Okada, K. Kurai, Y. Akahane, Y.
Sugai, T. Tanaka, K. Sato, F. Tsuda, Y. Miyamura, and M. Mayumi, J.
Gen. Virol. 73:673-679, 1992) and this new genotyping system.
Comparison of the results showed concordant results for 539 samples
(88.8%). Of the 68 samples with discordant results, the nucleotide
sequences of the HCV isolates were determined in 23, and their
genotypes were determined by molecular evolutionary analysis, In
all 23 samples, the assignment of genotype by our new genotyping
system was correct. This genotyping system may be useful for
large-scale determination of HCV genotypes in clinical studies.
Author: OHNO T, UNIV FLORIDA, DEPT MED, SECT HEPATOBILIARY
DIS, DIV GASTROENTEROL HEPATOL & NUTR, GAINESVILLE, FL
32610
Source: JOURNAL OF CLINICAL MICROBIOLOGY 1997
JAN;35(1):201-207
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